TLÜ ÕIS

Cloning and Expression in Prokaryotes

Course code

MLK7112.LT

old course code

Course title in Estonian

Kloonimine ja ekspressioon prokarüootides

Course title in English

Cloning and Expression in Prokaryotes

ECTS credits

6.0

Assessment form

Examination

lecturer of 2025/2026 Spring semester

Not opened for teaching. Click the study programme link below to see the nominal division schedule.

lecturer of 2026/2027 Autumn semester

Not opened for teaching. Click the study programme link below to see the nominal division schedule.

Course aims

To provide knowledge about the most important steps of molecular cloning and expression in prokaryotes.

Brief description of the course

Cloning steps and components, restriction enzymes, cDNA synthesis, gene synthesis, polymerase chain reaction (PCR), primer design, RNA and DNA analysis, DNA sequencing, BLAST, transformation, fusion protein expression, purification and analysis, peptide analysis and concentration determination.

Learning outcomes in the course

Upon completing the course the student:
- is able to design a primer and can practically carry out the main steps of molecular cloning;
- is able to find and analyze potential sources of error in the cloning process;
- can express and purify fusion proteins on a large scale using affinity chromatography and analyze the process.

Teacher

Stela Stefanova Koch PhD

Prerequisite course 1

Genetics (MLB6013.LT)

Prerequisite course 2

Biochemistry (MLK6922.LT)

The course is a prerequisite

Cloning and Expression in Eucaryotes (MLK7109.LT)

Study programmes containing that course