Recombinant Peptides and Proteins: Expression of Fusion Proteins in E.coli
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Course code
MLK7068.LT
old course code
MLK7068
Course title in Estonian
Rekombinantsed peptiidid ja valgud: fusioonvalkude ekspressioon E.coli-s
Course title in English
Recombinant Peptides and Proteins: Expression of Fusion Proteins in E.coli
ECTS credits
3.0
Assessment form
Examination
lecturer of 2023/2024 Spring semester
Not opened for teaching. Click the study programme link below to see the nominal division schedule.
lecturer of 2024/2025 Autumn semester
Not opened for teaching. Click the study programme link below to see the nominal division schedule.
Course aims
Elective (open); to pass on the knowledge about expression of fusion proteins in E.coli, recombinant product purification and analysis.
Brief description of the course
Preparation of competent cells, transformation, fusion protein expression, purification and analysis.
Learning outcomes in the course
Upon completing the course the student:
Student is capable to express and purify fusion proteins with affinity chromatography and analyse process and results.
Teacher
MSc Margit Kaldmäe
Additional information
Lectures are obligatory – there is no possibility to attend in practical work without 100% participation.
Parctical work is obligatory (100%).
Working students most likely have to make some changes in their work schedule – usually otherwise it is hard to pass given course.
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